Several assays have been used to assess the total antioxidant content of foods, e.g. the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) equivalent antioxidant capacity (TEAC) assay [12], the ferric-reducing ability of plasma (FRAP) [13] and the oxygen radical absorbance capacity assay (ORAC) assay [14]. Based on careful considerations (see Blomhoff 2005 and Halvorsen et al 2002 for discussion [15,16]) we chose to use a modified version of the FRAP assay by Benzie and Strain [13] for total antioxidant analysis [16]. Most importantly, the modified FRAP assay is a simple, fast and inexpensive assay with little selectivity. Assay conditions, such as extraction solvents, were optimized regarding detection of both lipophilic and hydrophilic antioxidants [16]. The FRAP assay directly measures antioxidants with a reduction potential below the reduction potential of the Fe3+/Fe2+ couple [16,17]. Thus, the FRAP assay does not measure glutathione. Most other assays have higher reduction potentials and measures glutathione and other thiols [18]. This may be an advantage when using the FRAP assay, because glutathione is found in high concentrations in foods but it is degraded in the intestine and poorly absorbed by humans [19]. A disadvantage of the FRAP assay is its inability to detect other small molecular weight thiols and sulfur containing molecules of e.g. garlic. Most assays for assessing total antioxidant capacity generally result in similar ranking of foods [20-23]. We have now performed a systematic measurement of the total antioxidant content of more than 3100 foods. This novel Antioxidant Food Table enables us to calculate total antioxidant content of complex diets, identify and rank potentially good sources of antioxidants, and provide the research community with comparable data on the relative antioxidant capacity of a wide range of foods.

Juicy and tart Montmorency cherries are your best source of the antioxidant melatonin. Other cherries have it, too, but Montmorency has the most. Melatonin protects the skin against ultraviolet radiation. Researchers have discovered that this powerful little nutrient also helps repair sunburned skin, since 
it stimulates new skin-cell growth. Cherries are also packed with vitamin C, which is needed to build collagen—your skin’s natural “scaffolding” and a wrinkle preventer. Here are some more foods proven to fight wrinkles (and two you should avoid).
Sugar and refined carbohydrates are considered 'empty calories', mainly because they don't contain any useful nutrients. Some foods high in sugar are heavily processed, and the sweet stuff is added to make them more palatable and desirable. It's these foods that are not always easy to spot the sugar in, and often those that are marketed as 'healthy', or low in fat which can be the worst offenders. In some cases, ready meals could contain up to 12 teaspoons of sugar per portion, a can of cola houses six and a bowl of dry Bran Flakes has three.
Sugar isn’t the only cause of cavities, though. Any carbohydrates, such as bread, potatoes and fruit, can help create the acid, but sugar is definitely a major player in the development of cavities. Many sugary treats, such as soda, cookies and candy, stay on the teeth for longer periods because they aren’t easily removed by your saliva. That extended time on your

Can't remember where you put your keys or why you walked into a particular room? Chocolate may help: Recent research suggests that antioxidants called flavanols found in cocoa can helpimprove function in the area of the brain responsible for this type of age-related memory loss. Participants in the study were placed on a special diet high in raw cocoa flavanols called epicatechin. At the end of the three-month period they scored significantly higher on memory tests than the control group.
There’s no doubt that dark chocolate is trending in today’s marketplace, and sales don’t seem to be slowing down anytime soon. Over the last few years, the chocolate industry has seen a move to premium and certified organic dark chocolate, specifically products that are single-origin; have high cacao content’ use natural sweeteners, such as agave, stevia, yacon or coconut sugar; as well as increased sustainable sourcing and origin labeling. As science shows more and more benefits of dark chocolate, its popularity will only continue to grow.
The research included information that animal studies have found the hippocampus, which is an area in the brain associated with memory, may be affected by refined sugar. Two studies were conducted in the published report. In the first study, participants that self-reported eating a high-sugar diet had poorer performance on hippocampal related memory tasks. In the second study, the results were replicated. The second study also revealed that the effect of high sugar consumption on memory appears to be directly related to the hippocampal region and no other areas which may also affect memory, such as the prefrontal cortex.
One of the components found in dark chocolate is theobromine. Theobromine is structurally quite similar to caffeine, its sister chemical. Theobromine, when consumed in larger amounts, can cause a dip in blood pressure, excitability and give energy. This energy can be followed by a crash, leading some critics to tout chocolate as a dangerous addictive substance.

One of the effects of sugar on the body is that it could make your depression worse, according to research. “I like to tell my patients there’s a truth to the saying, ‘You are what you eat,’” says psychologist Deborah Serani, PsyD, award-winning author of Depression in Later Life. “High levels of sugar in the form of simple carbohydrates leads to spikes and crashes in glucose levels, which can worsen mood, increase irritability, agitation, irregular sleeping, and increase inflammation.” Instead, munch on lean protein, complex carbs, and foods with omega-3s, folate, and B vitamins.
Fructose—the sugar that naturally occurs in fruit and is a component, with glucose, of high fructose corn syrup (HFCS) and table sugar—lights up the brain's reward center, says pediatric endocrinologist Robert Lustig, MD, of UCSF Benioff Children's Hospital in San Francisco. But over time, a diet packed with fructose (especially from HFCS) can make it tougher to learn and remember, animal research suggests. To stay in peak mental shape, try sticking with savory snacks.
Because refined dietary sugars lack minerals and vitamins, they must draw upon the body's micro-nutrient stores in order to be metabolized into the system. When these storehouses are depleted, metabolization of cholesterol and fatty acid is impeded, contributing to higher blood serum triglycerides, cholesterol, promoting obesity due to higher fatty acid storage around organs and in sub-cutaneous tissue folds.

Just empty and quickly digested calories that actually pull minerals from the body during digestion. It creates a hormone cascade when consumed that starts a positive feedback loop in the body to encourage more consumption. In a time when food was scarce and needed to be contained in large amounts in the summer when available to survive the winter, this was a good thing. In today’s world of constant access to processed foods, this natural biological purpose highlights one of the negative effects of sugar. Here’s why:

So what did the study show? The researchers found that the flavanol content of cocoa powder (30.1 milligrams per gram) was significantly greater than all of the other super fruit powders. It was also revealed that dark chocolate’s antioxidant capacity was higher than all of the super fruit juices except pomegranate. The total polyphenol content per serving was also highest for dark chocolate (about 1,000 milligrams per serving), which was significantly higher than all of the fruit juices except pomegranate juice. (11)